Monday, April 11, 2011

Tracing Our Ancestry From Our Mitochondrial DNA Lab Discussion

A) In this experiment, our lab table was successful in completing the experiment. The gel and markings on it turned out to be fine, and the results proved our incredible success. After the errors of our last lab experiment which led to the burning of our gel (results) we worked together as a lab table to ensure that this experiment worked out and that our mitochondrial DNA was ready for sequencing after the recent extraction. All four of the visible bands were in the desired location indicating the success and glory of performing the experiment correctly.

B) Sources of Error:
- We could have ran the gel for too long and burned off our half again.
- Could have not rinsed mouth thoroughly prior to cheek cell extraction and sequenced the mitochondrial DNA of a chicken or a strawberry.
- Chelex beads missed the DNASE and the DNA was destroyed before sequencing took place.
- No cheek cells were extracted and there was not sufficient mitochondrial DNA for successful extraction.

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